출판사 : cold spring

출판년도 : 2013년

ISBN : 9781936113019



This 4th edition of the "Mouse Manual"-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on:

· generation of induced pluripotent stem cells
· RNA microinjections
· lentiviral microinjections and infection
· assisted reproduction techniques for sperm and embryo cryopreservation
· isolation, generation, and transplantation of spermatogonial stem cell lines
· in utero electroporation of gene constructs into postimplantation embryos
· vibratome sectioning of live and fixed tissues for imaging thick tissue sections
· whole-mount fluorescent staining methods for three-dimensional visualization

In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.



Table Of Contents

CHAPTER 1 Genetics and Embryology of the Mouse: Past, Present, and Future
CHAPTER 2 Summary of Mouse Development
CHAPTER 3 A Mouse Colony for the Production of Transgenic and Chimeric Animals
CHAPTER 4 Recovery and In Vitro Culture of Preimplantation Embryos
CHAPTER 5 Isolation, Culture, and Manipulation of Postimplantation Embryos
CHAPTER 6 Surgical Procedures
CHAPTER 7 Production of Transgenic Mice by Pronuclear Microinjection
CHAPTER 8 Embryo-Derived Stem Cell Lines
CHAPTER 9 Germline-Competent Stem Cells Derived from Adult Mice
CHAPTER 10 Vector Designs for Pluripotent Stem Cell - Based Transgenesis and Genome Alterations
CHAPTER 11 Introduction of Foreign DNA into Embryonic Stem Cells
CHAPTER 12 Production of Chimeras 
CHAPTER 13 Genotyping 
CHAPTER 14 Parthenogenesis, Pronuclear Transfer, and Mouse Cloning 
CHAPTER 15 Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial
Insemination, and Intracytoplasmic Sperm Injection 
CHAPTER 16 Cryopreservation, Rederivation, and Transport of Mouse Strains 
CHAPTER 17 Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems
CHAPTER 18 Setting Up a Micromanipulation Laboratory

APPENDIX