Equine Reproductive Procedures, 2nd Edition

John Dascanio (Editor), Patrick McCue (Editor)
ISBN: 9781119555988
March 2021
Wiley-Blackwell
784 Pages

Description

The second edition of Equine Reproductive Procedures provides equine practitioners, veterinary students, and equine theriogenologists with a fully updated, practical guide to techniques in equine reproductive practice. This easy-to-use resource covers skills-based information with a clinical focus, taking an easy-to-follow step-by-step approach.

The book covers topics related to the reproductive management of horses, including diagnostic techniques and therapeutic procedures for stallions, mares, and foals. Procedures highlighted in the book are supported with clear descriptions and photographs. Readers will also find a list of required supplies for the procedure and a reference list.

• Provides step-by-step descriptions of techniques related to equine theriogenology
• Serves as a valuable practice tool
• Offers 39 new techniques not found in the first edition
• Includes key updates to existing techniques

 Those studying equine reproduction and specialists in theriogenology will find this an essential ‘how-to’ guide for their practice library.

Table of contents

1 Reproductive Evaluation of the Mare
Patrick M. McCue

2 Teasing
Patrick M. McCue

3 Tail Wrap and Preparation/Washing of the Perineum
John J. Dascanio

4 Placement of a Tail Rope
John J. Dascanio

5 Perineal Conformation Evaluation
John J. Dascanio

6 Removal of a Persistent Hymen
John J. Dascanio

7 Palpation of the Reproductive Tract of the Non-Pregnant Mare
Patrick M. McCue

8 Ultrasound Evaluation of the Non-Pregnant Mare
Patrick M. McCue

9 Prediction of Ovulation
Patrick M. McCue

10 Speculum Examination of the Vagina
John J. Dascanio

11 Digital Examination of the Vagina/Cervix
Sofie Sitters

12 Uterine Culture Collection: Swab/Brush
John J. Dascanio

13 Antimicrobiotic Sensitivity Testing
Jillian Bishop and Patrick M. McCue

14 Microbiology: Microbial Culture
Patrick M. McCue and Jillian Bishop

15 Microbiology: Gram Stain
Jillian Bishop and Patrick M. McCue

16 qPCR Assay for the Diagnosis of Infectious Endometritis
Ryan A. Ferris and Patrick M. McCue

17 Uterine Cytology Collection: Swab/Brush
John J. Dascanio and Ryan A. Ferris

18 Uterine Culture/Cytology: Low Volume Lavage
John J. Dascanio

19 Endometrial Biopsy
Patrick M. McCue

20 Hysteroscopic Examination of the Uterus
Patrick M. McCue

21 Endometrial Cyst Removal
John J. Dascanio

22 Chromosomal Analysis
Terje Raudsepp

23 Endocrinological Examination of the Mare
Patrick M. McCue

24 Laparoscopic Examination of the Uterus and Ovaries
Dean A. Hendrickson

25 Evaluation of the Mammary Gland
Patrick M. McCue

26 Antisperm Antibody Testing
Patrick M. McCue

27 Starch Granule Test for the Evaluation of Oviductal Patency
Sofie Sitters and John J. Dascanio

28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency
Sofie Sitters and John J. Dascanio

29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency
Sofie Sitters and Patrick M. McCue

30 Prostaglandin E2 Treatment for Blocked Oviducts
Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

31 Prostaglandin E1 Treatment for Blocked Oviducts
Patrick M. McCue and Charles F. Scoggin

32 Hysterocoscopic Hydrotubation of the Oviducts
Charles F. Scoggin

33 Transvaginal Ovarian Biopsy
Claire E. Card

34 Uterine Lavage
John J. Dascanio

35 Uterine Infusion
John J. Dascanio

36  Therapeutics for Infectious Endometritis
Ryan A. Ferris

37 Bacterial Biofilm and Endometritis
Ryan A. Ferris

38 Latent Uterine Bacterial Infections.
Morten Rønn Petersen and Anders Miki Bojesen

39 Acupuncture in Mare Reproduction
Kristina Lu and Lauren Javernick

40 Restraint for Breeding
John J. Dascanio

41 Natural Service: Pasture Breeding
Patrick M. McCue

42 Natural Service: In-hand Breeding
Charles F. Scoggin

43 Breeding Stitches
Charles F. Scoggin

44 Reinforcement Breeding
Charles F. Scoggin

45 Breeding with Fresh or Cooled Semen
John J. Dascanio and Patrick M. McCue

46 Insemination Through a Vaginal Speculum
Patrick M. McCue

47 Breeding with Frozen Semen
John J. Dascanio

48 Deep Horn Insemination
John J. Dascanio

49 Hysteroscopic (Low Dose) Insemination
Patrick M. McCue

50 Transvaginal oocyte pickup (OPU)
Jennifer N. Hatzel

51 Packaging and Transport of Oocytes
Jennifer N. Hatzel

52 Intracytoplasmic Sperm Injection (ICSI)
Jennifer N. Hatzel and JoAnne Stokes

53 Caslick Operation or Vulvoplasty
John J. Dascanio

54 Contagious Equine Metritis Testing
John J. Dascanio

55 Management of Seasonal Anestrus: Artificial Photoperiod
Patrick M. McCue

56 Management of Seasonal Anestrus: Hormone Therapy
Patrick M. McCue

57 Hormone Therapy in Cycling Mares
Patrick M. McCue

58 Estrus Suppression
Patrick M. McCue

59 Intra-uterine Devices for Estrus Suppression
Carlos M. Gradil

60 Immunocontraception
Patrick M. McCue

61 Harvesting and Shipping Ovaries for Oocyte Recovery
Patrick M. McCue

62 Embryo Collection
Patrick M. McCue

63 Embryo Evaluation
Patrick M. McCue

64 Embryo Biopsy
Patrick M. McCue

65 Embryo Packaging for Cooled Transport
Patrick M. McCue

66 Embryo Cryopreservation
Patrick M. McCue

67 Non-Surgical Embryo Transfer
Patrick M. McCue

68 Autogenous Transfer of ICSI Derived Embryos
Jennifer N. Hatzel

69 Palpation of the Pregnant Mare Per Rectum
Sofie Sitters

70 Ultrasound Examination of the Pregnant Mare
Patrick M. McCue

71 Speculum Examination of the Pregnant Mare
Sofie Sitters

72 Determination of Equine Fetal Sex 55 – 200 days
Richard D. Holder

73 Assessment of Late Term Fetal Well-being
John J. Dascanio

74 Combined Thickness of the Uterus and Placenta
Patrick M. McCue

75 Placentitis – Diagnosis and Treatment
Justin W. McNaughten and Margo L. Macpherson

76 Doppler Ultrasonography
John J. Dascanio

77 Prediction of Fetal Age
John J. Dascanio

78 Twin Reduction: Manual Technique
John J. Dascanio

79 Twin Reduction: Transvaginal Aspiration
John J. Dascanio

80 Twin Reduction:  Cranio-Cervical Dislocation
Karen Wolfsdorf

81 Twin Reduction: Thoracic Compression
Patrick M. McCue

82 Twin Reduction: Transabdominal Fetal Cardiac Puncture
John J. Dascanio

83 Elective Termination of Pregnancy
John J. Dascanio

84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission
Julie T. Cecere and John J. Dascanio

85 Endocrine Evaluation of Pregnancy
Patrick M. McCue

86 Treatment of Vaginal Varicosities
John J. Dascanio

87 Manual Correction of Uterine Torsion
John J. Dascanio

88 Preparation of the Mare for Foaling
John J. Dascanio

89 Assessment of Mammary Gland Secretions to Predict Foaling
John J. Dascanio

90 Assessment of pH of Mammary Gland Secretions to Predict Foaling
Patrick M. McCue

91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras
Ryan A. Ferris

92 Foaling Alert: Vulvar Device
John J. Dascanio

93 Positional Labor Alert Devices
Ryan A. Ferris

94 Induction of Parturition
John J. Dascanio

95 Normal Foaling
John J. Dascanio

96 Premature Separation of the Placenta
Patrick M. McCue

97 Dystocia Management
Patrick M. McCue and Sofie Sitters

98 Dystocia Correction
John J. Dascanio

99 Fetotomy
John J. Dascanio

100 Epidural
John J. Dascanio

101 Induction of Lactation to Create a Nurse Mare
Pouya Dini and Peter F. Daels

102 Screening the Pregnant Mare to Prevent Neonatal Isoerythrolysis
Patrick M. McCue

103 Evaluation of Colostrum Specific Gravity
Patrick M. McCue

104 Evaluation of Colostrum Quality: Brix Refractometry
Patrick M. McCue

105 Colostrum Banking
Patrick M. McCue

106 Obtaining Milk from the Mare
John J. Dascanio

107 Placental Evaluation
Patrick M. McCue

108 Removal of Retained Placenta
John J. Dascanio

109 Abdominocentesis in the Postpartum Mare
John J. Dascanio

110 Uterine Prolapse Treatment
John J. Dascanio

111 Buhner Needle Placement of a Perivulvar Suture
John J. Dascanio

112 Breeding Soundness Evaluation
Patrick M. McCue

113 Training of a Stallion to Use a Phantom
John J. Dascanio

114 Missouri Artificial Vagina
John J. Dascanio  and Lynda M.J. Miller

115 Colorado Model Artificial Vagina
Patrick M. McCue

116 Roanoke Artificial Vagina
John J. Dascanio

117 Hannover Artificial Vagina
Sofie Sitters

118 Teaser Stallions
Charles F. Scoggin

119 Standing Semen Collection
John J. Dascanio

120 Collection of Semen using an Open-Ended Artificial Vagina
Patrick M. McCue

121 Chemical Ejaculation
Patrick M. McCue

122 Preparation of a Jump Mare or a Mare for Natural Cover
John J. Dascanio

123 Washing the Penis
John J. Dascanio

124 Evaluation of Sexual Behavior in the Stallion
John J. Dascanio Sue M. McDonnell

125 Enhancement of Sexual Interest and Response in Stallions
Sue M. McDonnell

126 Management of Ejaculation Difficulty in Stallions
Sue M. McDonnell

127 Calibrated Spectrophotometer Evaluation of Sperm Concentration
John J. Dascanio

128 Hemocytometer Evaluation of Sperm Concentration
John J. Dascanio

129 NucleoCounter® Evaluation of Sperm Concentration and Viability
Patrick M. McCue

130 Visual Evaluation of Sperm Motility
Patrick M. McCue

131 Computer-Assisted Sperm Analysis
John J. Dascanio  and Lynda M. J. Miller

132 Eosin-Nigrosin Staining in the Evaluation of Sperm
Julie T. Cecere

133 Evaluation of Sperm Morphology
Leonardo Brito

134 Determination of Daily Sperm Output
Patrick M. McCue

135 Measurement of Testicular Size and Estimation of Daily Sperm Output
Patrick M. McCue

136 Wet Mount Evaluation of Sperm
John J. Dascanio

137 Diff-Quik  Evaluation of Round Cells and Sperm
John J. Dascanio and Lynda M. J. Miller

138 Electron Microscopy of Constituents of Seminal Ejaculates
D. N. Rao Veeramachaneni, BVSc, MScVet, PhD

139 Bacterial Culture in the Stallion
John J. Dascanio

140 Evaluation of pH and Osmolarity of Semen
Patrick M. McCue

141 Alkaline Phosphatase: A Marker for Ejaculation
Patrick M. McCue

142 Diagnosis and Management of Urospermia
John J. Dascanio

143 Hemospermia
Patrick M. McCue

144 Semen Extenders and Sperm Media
Patrick M. McCue

145  Preparation of Semen for Cooled Transport
Etta A. Bradecamp

146 Packing Semen for Cooled Transport
Etta A. Bradecamp

147 Longevity Testing of Sperm
John J. Dascanio

148 Calculation of g Force for Centrifuging Semen
John J. Dascanio

149 Centrifugation of Semen: Standard Technique
Etta A. Bradecamp

150 Centrifugation of Semen: Cushion Technique
Etta A. Bradecamp

151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique
Etta A. Bradecamp

152 Use of a Sperm Filter
Marco A. Alvarenga, Lorenzo G. T. M. Segabinazzi

153 Semen Freezing
Patrick M. McCue

154 Refreezing Stallion Semen
Patrick M. McCue

155 Thawing Frozen Semen
John J. Dascanio

156 Semen Processing for ICSI
Patrick M. McCue

157 Preparing a Vapor Shipper
Julie T. Cecere and Paul Loomis

158 Loading a Vapor Shipper
Julie T. Cecere and Paul Loomis

159 Maintaining a Long-Term Frozen Semen Storage Tank
John J. Dascanio

160 International Transport of Frozen Semen
Paul Loomis

161 Harvesting and Shipping Testes
John J. Dascanio

162 Epididymal Sperm Recovery
John J. Dascanio  and Lynda M.J. Miller

163 Endoscopic Examination of the Urethra
Patrick M. McCue

164 Endoscopic Guided Cannulation of the Seminal Vesicles
Patrick M. McCue

165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord
Sofie Sitters

166 Palpation and Ultrasonography of the Accessory Sex Glands
Sofie Sitters

167 Radiographic Examination of the Penis
Patrick M. McCue

168 Testicular Biopsy and Aspiration
John J. Dascanio

169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14
Amanda I. Glazar  and Patrick M. McCue

170 Sperm Chromatin Structure Assay
Charles Love

171 Hypo-Osmotic Swelling Test
Patrick M. McCue

172 Assessment of Sperm Acrosomal Status: FITC PNA
Amanda I. Glazar and Patrick M. McCue

173 Assessment of Sperm Mitochondrial Function: JC-1 and Rhodamine 123
Amanda I. Glazar and Patrick M. McCue

174 Equine Viral Arteritis Testing
G. Reed Holyoak and Udeni B. R. Balasuriya

175 Diagnostic Endocrinology: Baseline Hormone Levels
Patrick M. McCue

176 Diagnostic Endocrinology: GnRH Stimulation Tests
Patrick M. McCue

177 Diagnostic Endocrinology: hCG Stimulation Test
Patrick M. McCue

178 Diagnostic Endocrinology: Estrogen Conjugate Assay
Patrick M. McCue

179 Diagnostic Tests for Cryptorchidism
Patrick M. McCue

180 Probang Apparatus
Julie T. Cecere

181 Support Apparatus for Paraphimosis
John J. Dascanio

182 Equine Castration Techniques
Stacy Anderson

183 Stallion Acupuncture
Rhonda A Rathgeber

184 Birth Resuscitation
Patrick M. McCue

185 Foal Handling and Restraint
Undine Christmann

186 Madigan Foal Squeeze Technique
John Madigan

187 Evaluation of Passive Transfer
Patrick M. McCue

188 Colostral Administration via Naso-Gastric Intubation
John J. Dascanio

189 Sedation of the foal
Undine Christmann

190 Intravenous Catheter Placement in the Foal
Undine Christmann

191 Plasma Therapy in the Foal
Undine Christmann

192 Acupuncture in the Neonate
Peter Morresey

193 Routine Care
Patrick M. McCue

194 Foal Rejection
Patrick M. McCue

195 Fostering a Foal onto a Nurse Mare
Peter F Daels and Pouya Dini

196 Entropion
John J. Dascanio

197 Umbilical Ultrasound in the Foal
Undine Christmann

198 Management of Soft Tissue Laxity in Foals
Stacy Anderson

199 Management of Angular Limb Deformities
Stacy Anderson

200 Management of Congenital Flexural Limb Deformities
Stacy Anderson

About the Author

JOHN DASCANIO, VMD, Diplomate ACT, Diplomate ABVP (Equine) is the Senior Associate Dean for Academic and Student Affairs and a Professor of Theriogenology at the Texas Tech University School of Veterinary Medicine in Amarillo, Texas, USA and was named the 2018 Theriogenologist of the Year by the American College of Theriogenologists.

PATRICK MCCUE, DVM, PhD, Diplomate ACT is the Iron Rose Ranch Professor of Equine Reproduction at Colorado State University College of Veterinary Medicine and Biomedical Sciences in Fort Collins, Colorado, USA and was named the 2017 Theriogenologist of the Year by the American College of Theriogenologists.